Difference between revisions of "Part:BBa K546005"

 
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<partinfo>BBa_K546005 short</partinfo>
 
<partinfo>BBa_K546005 short</partinfo>
  
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in progress
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This BioBrick composite part has an, colony wide, initially increasing and finally constant Green Fluorescent Protein (GFP) with a LVA Tag output.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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LuxR is slightly produced by the sub-[https://parts.igem.org/Part:BBa_F2621 BioBrick part] F2621 at the beginning. Meanwhile also the mildly transcribed BioBrick part C0061 creates an increase in the [http://en.wikipedia.org/wiki/Autoinducer autoinducer] 3OC6HSL. As the LuxR and the autoinducer are formed simultaneously, a complex of these two forms -in all the cells of a colony. This complex initiates a positive and two times a negetive feedback loop. The gene luxI is part of the positive feedback loop and will be transcribed more often (resulting in a greater autoinducer production), but the promoter in front of the luxr gene will down regulate the formation of LuxR. The increase in and finally more or less equilibrium state of the LuxR-autoinducer complex is monitored by the Green Fluorescent Protein with a LVA Tag.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K546005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K546005 SequenceAndFeatures</partinfo>
  

Revision as of 20:14, 19 September 2011

Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).

This BioBrick composite part has an, colony wide, initially increasing and finally constant Green Fluorescent Protein (GFP) with a LVA Tag output.


Usage and Biology

LuxR is slightly produced by the sub-BioBrick part F2621 at the beginning. Meanwhile also the mildly transcribed BioBrick part C0061 creates an increase in the [http://en.wikipedia.org/wiki/Autoinducer autoinducer] 3OC6HSL. As the LuxR and the autoinducer are formed simultaneously, a complex of these two forms -in all the cells of a colony. This complex initiates a positive and two times a negetive feedback loop. The gene luxI is part of the positive feedback loop and will be transcribed more often (resulting in a greater autoinducer production), but the promoter in front of the luxr gene will down regulate the formation of LuxR. The increase in and finally more or less equilibrium state of the LuxR-autoinducer complex is monitored by the Green Fluorescent Protein with a LVA Tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3909
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3189