Difference between revisions of "Part:BBa K546000:Design"

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===Design Notes===
 
===Design Notes===
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The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: [https://parts.igem.org/Part:BBa_K546003 K546003].
+
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device (R0063-B0034-C0062-B0010-B0012-R0062) turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: [https://parts.igem.org/Part:BBa_K546003 K546003].
  
 
===Source===
 
===Source===

Revision as of 18:31, 16 September 2011

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


Design Notes

The original R0063 BioBrick part (promoter) of the F2621 BioBrick device (R0063-B0034-C0062-B0010-B0012-R0062) turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: K546003.

Source

The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.

References