Difference between revisions of "Part:BBa K511800"
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This MammoBlock composite device produces the enhanced blue fluorescent protein EBFP2 at a low, constitutive level. | This MammoBlock composite device produces the enhanced blue fluorescent protein EBFP2 at a low, constitutive level. | ||
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| + | Figure 1 below show blue EBFP2 fluorescence driven by Hef1a; Figure 2 shows a bright field of the same culture. | ||
<html><img src='https://static.igem.org/mediawiki/parts/f/f3/MITBlue1.png | <html><img src='https://static.igem.org/mediawiki/parts/f/f3/MITBlue1.png | ||
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<html><img src='https://static.igem.org/mediawiki/parts/4/49/MITBlue2.png | <html><img src='https://static.igem.org/mediawiki/parts/4/49/MITBlue2.png | ||
' style="width:30%"><br></html> | ' style="width:30%"><br></html> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 04:26, 29 September 2011
Blue Fluorescent Protein Generator (Hef1a-EBFP2) MammoBlock Device
This MammoBlock composite device produces the enhanced blue fluorescent protein EBFP2 at a low, constitutive level.
Figure 1 below show blue EBFP2 fluorescence driven by Hef1a; Figure 2 shows a bright field of the same culture.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127 - 1000COMPATIBLE WITH RFC[1000]