Difference between revisions of "Part:BBa K511004:Design"
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===Source=== | ===Source=== | ||
| − | This part is a synthetic promoter formed from the minimal transcriptional sequence necessary for the immediate-early cytomegalovirus (CMV) promoter, a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator, and six tetracycline response elements (TREs) isolated from Escherichia coli. | + | This part is a synthetic promoter formed from the minimal transcriptional sequence necessary for the immediate-early cytomegalovirus (CMV) promoter, a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator, and six tetracycline response elements (TREs) isolated from Escherichia coli. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team. |
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===References=== | ===References=== | ||
Latest revision as of 01:01, 21 October 2011
TRE-Tight-LacOid Promoter MammoBlock
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 34
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 34
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 34
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Not applicable.
Source
This part is a synthetic promoter formed from the minimal transcriptional sequence necessary for the immediate-early cytomegalovirus (CMV) promoter, a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator, and six tetracycline response elements (TREs) isolated from Escherichia coli. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.