Difference between revisions of "Part:BBa K511002:Design"
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===Source=== | ===Source=== | ||
− | This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22. | + | This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team. |
===References=== | ===References=== |
Latest revision as of 01:00, 21 October 2011
minCMV-4xMnt Promoter MammoBlock
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 39
Illegal BglII site found at 110
Illegal BglII site found at 181
Illegal BglII site found at 252
Illegal XhoI site found at 1
Illegal XhoI site found at 72
Illegal XhoI site found at 143
Illegal XhoI site found at 214 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Not applicable.
Source
This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team.