Difference between revisions of "Part:BBa K511002:Design"

 
(Source)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K511002 short</partinfo>
 
<partinfo>BBa_K511002 short</partinfo>
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===Source===
 
===Source===
  
This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22.
+
This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team.
  
 
===References===
 
===References===

Latest revision as of 01:00, 21 October 2011

minCMV-4xMnt Promoter MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal BglII site found at 110
    Illegal BglII site found at 181
    Illegal BglII site found at 252
    Illegal XhoI site found at 1
    Illegal XhoI site found at 72
    Illegal XhoI site found at 143
    Illegal XhoI site found at 214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable.


Source

This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team.

References