Difference between revisions of "Part:BBa K427008"
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This part has no special safety considerations.
 
This part has no special safety considerations.
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
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===<partinfo>BBa_K427008 SequenceAndFeatures</partinfo>===
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<partinfo>BBa_K427008 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 11:32, 27 October 2010

Ter PBadweak Mu Mor Ter PM GFP cassette

This part was used for the characterization of the MuMor sensitivity tuner. It is composed of seven biobricks starting with Ter PBadweak, which is the part created by British Columbia team in 2007 starts with a terminator and a promoter that respond to high concentrations of Arabinose. It is followed by sequence coding for Mor protein which inter activates the Pm promoter and this enhance the PoPS activity. Then is followed by the GFP cassette sequence a biobrick.

This part has no special safety considerations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1501


References

BBa_F2620 Parts Registry entry with characterisation.

Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.