Difference between revisions of "Part:BBa K4183005"
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We incubated E. coli containing BBa_K4183005 overnight, and centrifuged 2 ml of the bacterial solution. We subsequently observed the supernatant under UV irradiation. The results showed (Figure 1) that the supernatant of the experimental group emitted green fluorescence compared with the control group without kp-sp (BBa_K2981006), demonstrating that GFP containing kp-sp was secreted outside the cell. | We incubated E. coli containing BBa_K4183005 overnight, and centrifuged 2 ml of the bacterial solution. We subsequently observed the supernatant under UV irradiation. The results showed (Figure 1) that the supernatant of the experimental group emitted green fluorescence compared with the control group without kp-sp (BBa_K2981006), demonstrating that GFP containing kp-sp was secreted outside the cell. | ||
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[[File:HS_China_improvement-1.png|900px|thumb|none|alt=Kp-Sp amilCP.|Figure 1. Secretion of GFP without (left)/with (right) addition of signal peptide (kp-sp).]] | [[File:HS_China_improvement-1.png|900px|thumb|none|alt=Kp-Sp amilCP.|Figure 1. Secretion of GFP without (left)/with (right) addition of signal peptide (kp-sp).]] | ||
Revision as of 14:34, 11 October 2022
Addition of kp-sp signal peptide at the N-terminal end of GFP
Addition of kp-sp signal peptide to the N-terminal end of GFP allows it to be secreted extracellularly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 966