Difference between revisions of "Part:BBa K4090007"
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[[File:T--SDSZ_China--csgA3.png|700px|thumb|center|Fig.2]]
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[[File:T--SDSZ_China--csgA3.png|700px|thumb|center|Fig.1]]
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[[File:T--SDSZ_China--csgAmfp52.png|700px|thumb|center|Fig.3]]
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[[File:T--SDSZ_China--csgAmfp52.png|700px|thumb|center|Fig.2]]
 
==Data==
 
==Data==
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[[File:T--SDSZ_China--csgA1.jpeg|700px|thumb|center|Fig.1]]
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[[File:T--SDSZ_China--csgA1.jpeg|700px|thumb|center|Fig.3]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:30, 17 October 2021


csgA-Mfp5-Mfp5

A kind of fusion protein that helps to form hydroxycalcium phosphate outside the membrane. Since the expression of a single Mfp5 gene is insufficient to cause a notable precipitation, our team transformed two mfp5 sequences into the plasmid.

Fig.1
Fig.2

Data

Fig.3

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 556
    Illegal BamHI site found at 784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

[1] DHAMI N K, REDDY M S, MUKHERJEE A. Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites[J]. Journal of Microbiology and Biotechnology, 2013, 23(5): 707-714.
[2] OKWADHA G D, LI J. Optimum conditions for microbial carbonate precipitation[J]. Chemosphere, 2010, 81(9): 1143-1148. [16]LI W, LIU L P, ZHOU P P, et al. Calcite precipitation induced by bacteria and bacterially produced carbonic anhydrase[J]. Current Science, 2011，100(4): 502-508.
[3] ANBU P, KANG C H, SHIN Y J, et al. Formations of calcium carbonate minerals by bacteria and its multiple applications[J]. Springerplus, 2016, 5(1): 1-26.
[4] Hale, L. V., Ma, Y. F. & Santerre, R. F. Semi-quantitative fuorescence analysis of calcein binding as a measurement of in vitro mineralization. Calcif. Tissue Int. 67, 80-84(2000). Ramachandran S K.
[5] Dick J, Windt W D, Graef B D, et al. Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species[J]. Biodegradation, 2016(4): 357-367.
[6] Qian C X, Chen H C, Ren L F, et al. Self-healing of early age cracks in cement-based materials by mineralization of carbonic anhydrase microorganism[J]. Frontiers in Microbiology, 2015(6): 1-9.