Difference between revisions of "Part:BBa K3892006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Added a BamHI site to the start of the protein to make it easier to change the promotor/RBS. Only a glycine is added which | + | Added a BamHI site to the start of the protein to make it easier to change the promotor/RBS. Only a glycine is added which did not impact folding. |
− | + | ||
Revision as of 08:19, 21 October 2021
J23110-G10 leader-EforRed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 75
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a BamHI site to the start of the protein to make it easier to change the promotor/RBS. Only a glycine is added which did not impact folding.
Source
EforRed (Echinopora forskaliana)