Difference between revisions of "Part:BBa K3846361"
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<partinfo>BBa_K3846361 short</partinfo> | <partinfo>BBa_K3846361 short</partinfo> | ||
| − | ATR2 expression | + | Since CYPs do not work without a reductase. Reductases are essential for every part containing CYP P450 enzymes. ATR2 from <i>Arabidopsis thaliana</i> has been shown to enable the efficient electron transfer for different CYP P450 enzymes. Aim of our project was to fuse CYPs with reductase but as a control the parallel expression of CYPs and reductase is nevertheless necessary. |
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| + | This phytobrick allows theIPTG-inducible expression of ATR2 under the T7 promoter. | ||
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<partinfo>BBa_K3846361 parameters</partinfo> | <partinfo>BBa_K3846361 parameters</partinfo> | ||
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| + | ===iGEM Hamburg 2021 part collection=== | ||
| + | Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. | ||
| + | Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry. | ||
| + | |||
| + | Fortunately we were able to change that and designed a novel golden gate based toolbox which allows. | ||
| + | <ol style="list-style-type:lower-alpha"> | ||
| + | <li>production of terpenoid precursors and simple terpenoids</li> | ||
| + | <li>creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products</li> | ||
| + | <li>modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)</li> | ||
| + | </ol> | ||
| + | |||
| + | ===MoClo-based Part Design 2.0=== | ||
| + | <p>To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. | ||
| + | More useful information and an overview of all our parts can be found on our [https://2021.igem.org/Team:Hamburg/Part_Collection wiki]. | ||
| + | |||
| + | [[File:T--Hamburg--parts overview MolClo.png|600px|thumb|left|'''Figure 1''': <b> MoClo syntax of the part collection. </b> <br>]] | ||
Latest revision as of 03:37, 20 October 2021
ATR2 expression casette (T7 promoter)
Since CYPs do not work without a reductase. Reductases are essential for every part containing CYP P450 enzymes. ATR2 from Arabidopsis thaliana has been shown to enable the efficient electron transfer for different CYP P450 enzymes. Aim of our project was to fuse CYPs with reductase but as a control the parallel expression of CYPs and reductase is nevertheless necessary.
This phytobrick allows theIPTG-inducible expression of ATR2 under the T7 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
iGEM Hamburg 2021 part collection
Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.
Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.
- production of terpenoid precursors and simple terpenoids
- creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products
- modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)
MoClo-based Part Design 2.0
To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. More useful information and an overview of all our parts can be found on our wiki.
