Difference between revisions of "Part:BBa K3796207"

Revision as of 02:33, 21 October 2021

Patp2-rbs-gfp-terminator

This part is composed of an inducible promoter Patp2, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3796213). P-atp2 is an alkali inducible promoter found in Corynebacterium glutamicum. Under high pH, the gfp will have higher expression, leading to stronger fluorescence intensity.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 14
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 14
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 14
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 14
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 14
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3796207 short</partinfo>
-This part is composed of an inducible promoter Patp2, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3568009). P-atp2 is an alkali inducible promoter found in Corynebacterium glutamicum. Under high pH, the gfp will have higher expression, leading to stronger fluorescence intensity.
+This part is composed of an inducible promoter Patp2, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3796213). P-atp2 is an alkali inducible promoter found in Corynebacterium glutamicum. Under high pH, the gfp will have higher expression, leading to stronger fluorescence intensity.
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