Difference between revisions of "Part:BBa K3796008"
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This composite part contains three coding sequences, capA,capB,capC, which are joined upstream and downstream of the promoter P0864+RBS and T7 terminator, respectively. It is used for the synthesis and extracellular transport of gamma-polyglutamic acid. | This composite part contains three coding sequences, capA,capB,capC, which are joined upstream and downstream of the promoter P0864+RBS and T7 terminator, respectively. It is used for the synthesis and extracellular transport of gamma-polyglutamic acid. | ||
| − | ==Usage== | + | ===Usage=== |
capA, capB and capC genes must be used together. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately. | capA, capB and capC genes must be used together. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately. | ||
| − | ==Biology== | + | ===Biology=== |
capABC gene is a gene cluster widely contained in <i>Bacillus</i> sp., which is also named pgsBCA gene cluster. | capABC gene is a gene cluster widely contained in <i>Bacillus</i> sp., which is also named pgsBCA gene cluster. | ||
capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free. | capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free. | ||
Revision as of 03:14, 21 October 2021
capABC for gamma-PGA synthesis
This composite part contains three coding sequences, capA,capB,capC, which are joined upstream and downstream of the promoter P0864+RBS and T7 terminator, respectively. It is used for the synthesis and extracellular transport of gamma-polyglutamic acid.
Usage
capA, capB and capC genes must be used together. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately.
Biology
capABC gene is a gene cluster widely contained in Bacillus sp., which is also named pgsBCA gene cluster. capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2046
Illegal NheI site found at 2487
Illegal NheI site found at 2491
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2046
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322
Illegal NgoMIV site found at 2679 - 1000COMPATIBLE WITH RFC[1000]