Difference between revisions of "Part:BBa K3796004"
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One of the essential genes for the synthesis of γ-PGA, also known as pgsC. | One of the essential genes for the synthesis of γ-PGA, also known as pgsC. | ||
| − | ==Usage and Biology== | + | ===Usage and Biology=== |
BBa_K3796004 is partially of the gene cluster to synthesize γ-PGA, in which capA, capB and capC genes must be used altogether. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately. | BBa_K3796004 is partially of the gene cluster to synthesize γ-PGA, in which capA, capB and capC genes must be used altogether. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately. | ||
Revision as of 03:34, 21 October 2021
capC from Bacillus subtilis str.168
One of the essential genes for the synthesis of γ-PGA, also known as pgsC.
Usage and Biology
BBa_K3796004 is partially of the gene cluster to synthesize γ-PGA, in which capA, capB and capC genes must be used altogether. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately.
capABC gene is a gene cluster widely contained in Bacillus sp., which is also named pgsBCA gene cluster. capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
- 1000COMPATIBLE WITH RFC[1000]