Difference between revisions of "Part:BBa K3796001"

 
 
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<partinfo>BBa_K3796001 short</partinfo>
 
<partinfo>BBa_K3796001 short</partinfo>
  
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The RBS sequence, or ribosome binding site, is located several sequences upstream of the gene expression frame and is an important sequence for pre-translational recognition and binding of the ribosome. When the DNA fragment downstream of the promoter and upstream of the terminator is transcribed out of the mRNA, the ribosome recognizes and binds the RBS sequence on the mRNA and then scans down until it recognizes the start codon and begins translation.
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This is a strong RBS in <i>C.glutamicum</i> that can be used to improve traslation efficiency. It is particularly useful in improving the yield of fermentation products in <i>C.glutamicum</i>(Shi F, et al., 2020).
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===Reference===
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[1]Shi F, Fan Z, Zhang S, et al. Optimization of ribosomal binding site sequences for gene expression and 4-hydroxyisoleucine biosynthesis in recombinant corynebacterium glutamicum[J]. Enzyme and Microbial Technology, 2020, 140: 109622.
  
 
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Latest revision as of 03:42, 21 October 2021


Corynebacterium glutamicum strong RBS

This is a strong RBS in C.glutamicum that can be used to improve traslation efficiency. It is particularly useful in improving the yield of fermentation products in C.glutamicum(Shi F, et al., 2020).

Reference

[1]Shi F, Fan Z, Zhang S, et al. Optimization of ribosomal binding site sequences for gene expression and 4-hydroxyisoleucine biosynthesis in recombinant corynebacterium glutamicum[J]. Enzyme and Microbial Technology, 2020, 140: 109622.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]