Difference between revisions of "Part:BBa K3598008"

Revision as of 06:24, 25 October 2020

Snake Cathelicidin-BF

Snake Cathelicidin-BF is an antimicrobial peptide purified from the snake venoms of Bungarus fasciatus. It is the first identified cathelicidin antimicrobial peptide in reptiles.

Usage and Biology

Figure 1. Part demonstration

The Snake Cathelicidin-BF coding part was the original sequence of Snake Cathelicidin-BF we sent to a synthetic company to produce our Snake Cathelicidin-BF AMP. We verified the antimicrobial potency of Snake Cathelicidin-BF purchased from the company by adding its solution and placing pieces of paper soaked with its solution to plates inoculated with P. acnes and E. coli MG1655. The results showed inhibition zones in areas of direct contact between bacteria and Snake Cathelicidin-BF, which means Snake Cathelicidin-BF is effective in killing both bacteria, and would not affect bacteria outside its contact.

Figure 3. Plate verification result of Snake Cathelicidin-BF's ability to kill P. acnes

Then, we verified Snake Cathelicidin-BF's ability to kill E. coli MG1655 and P. acnes by adding its solution to liquid culture inoculated with the two bacteria. We set the AMP concentration gradient as 0, 2, 4, 8, 12, 16 mg/L as a group, 0, 0.5, 1, 2, 3 mg/L as a group, and measured the OD600 of the E. coli through time. Since P. acnes has a slow growth rate, we only measured OD600 after 24 hours of cultivation. The results indicate that Snake Cathelicidin-BF's ability to kill E. coli MG1655 peaked at concentrations 3mg/L and 8mg/L at 8h and 24h, its effect reaching OD600 of about 0.15 and 0.025. Snake Cathelicidin-BF's ability to kill P. acnes peaked at concentration 16mg/L, its effect reaching OD600 of about 0.225.

Figure 4. OD600 verification result of Snake Cathelicidin-BF's ability to kill E. coli

Figure 5. OD600 verification result of Snake Cathelicidin-BF's ability to kill P. acnes

Sequence and Features