Difference between revisions of "Part:BBa K3544004:Design"

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===References===
 
===References===
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Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.

Latest revision as of 13:25, 27 October 2020


Csy4,Encoding endonuclease that specifically recognizes and cleaves 28nt stem-loop structure


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 27


Design Notes

Csy4 can specifically cleave the 28nt stem-loop structure to separate the target fragments before and after the stem-loop structure

Source

The original sequence was obtained from reference, and it's synthesized according to codon usage bias in S.c from Sangon

References

Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.