Difference between revisions of "Part:BBa K3544004:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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used to specifically cleave the 28nt stem-loop structure to separate the target fragments before and after the stem-loop structure
+
Csy4 can specifically cleave the 28nt stem-loop structure to separate the target fragments before and after the stem-loop structure
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 13:17, 27 October 2020


Csy4,Encoding endonuclease that specifically recognizes and cleaves 28nt stem-loop structure


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 27


Design Notes

Csy4 can specifically cleave the 28nt stem-loop structure to separate the target fragments before and after the stem-loop structure

Source

Pseudomonas aeruginosa

References