Difference between revisions of "Part:BBa K3544000:Design"

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===References===
 
===References===
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Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.

Revision as of 13:00, 27 October 2020


Coding sequence of BcABA1, a cytochrome P450s belong to biosynthetic genecluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1107


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze alpha-ionylideneethane into alpha-ionylideneacetic acid

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in S.c and synthesized from Sangon

References

Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.