Difference between revisions of "Part:BBa K3484005"
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This composite part is composed by all the following parts: | This composite part is composed by all the following parts: | ||
− | <html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>: A reliable double terminator that consists on BBa_B0010 and BBa_B0012. | + | <html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>:A reliable double terminator that consists on BBa_B0010 and BBa_B0012. |
<html><a href="https://parts.igem.org/Part:BBa_B0032">BBa_B0032</a></html>: A medium RBS. The RBS.3 (medium) (derivative of BBa_0030 | <html><a href="https://parts.igem.org/Part:BBa_B0032">BBa_B0032</a></html>: A medium RBS. The RBS.3 (medium) (derivative of BBa_0030 |
Revision as of 16:25, 26 October 2020
Bad promoter + LuxI
This construct synthesizes lactone via the LuxI enzyme. This enzyme is regulated by the pBad promoter, which is induced by Arabinose but repressed by Glucose. This means that the production of lactone can be controlled in two ways, increasing the production with Arabinose and decreasing it with Glucose.
A schematic representation of the interactions is shown below (Fig.1). Notice that when LuxI is formed, this enzyme produces lactone (AHL).
This system allows to have a control of the AHL lactone production in a really consistent way, and consequently, is a system that could be used to simulate other different ones. The composite part is Biobrick compatible and was characterized in E.Coli DH5α.
This composite part is composed by all the following parts:
BBa_B0015:A reliable double terminator that consists on BBa_B0010 and BBa_B0012.
BBa_B0032: A medium RBS. The RBS.3 (medium) (derivative of BBa_0030
BBa_I13453: PBad promoter from I0500 without AraC
BBa_C0061: LuxI gene
Model & Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 262
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 931
Illegal BamHI site found at 202 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]