Difference between revisions of "Part:BBa K3484004"

 
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<partinfo>BBa_K3484004 short</partinfo>
 
<partinfo>BBa_K3484004 short</partinfo>
  
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Reporter
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This composite part produces sfGFP when a large enough concentration of lactone is present in the medium. In this case,vit constitutively produces LuxR, which binds to the lactone present in the medium and together, they form a complex that activates the pLux promoter, generating the sfGFP whose fluorescence can be measured. The schematics of this composite part can be seen in Figure 1.
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[[File:UPF_Barcelona--Reporter1.png|600px|thumb|center|Figure 1. Scheme of the interactions on the AHL sfGFP+ASV tag reporter sensor cell.]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:11, 26 October 2020


AHL sfGFP+ASV reporter

This composite part produces sfGFP when a large enough concentration of lactone is present in the medium. In this case,vit constitutively produces LuxR, which binds to the lactone present in the medium and together, they form a complex that activates the pLux promoter, generating the sfGFP whose fluorescence can be measured. The schematics of this composite part can be seen in Figure 1.

Figure 1. Scheme of the interactions on the AHL sfGFP+ASV tag reporter sensor cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1142