Difference between revisions of "Part:BBa K3447101"
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===Usage and Biology=== | ===Usage and Biology=== | ||
In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.<br> | In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.<br> | ||
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| + | Arabinose induced RelE expression: <partinfo>BBa_K3447104</partinfo><br> | ||
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| + | AHL induced RelE expression: <partinfo>BBa_K3447106</partinfo><br> | ||
==<b>Design</b>== | ==<b>Design</b>== | ||
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We added some synonymous mutations to avoid part rules.<br> | We added some synonymous mutations to avoid part rules.<br> | ||
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Revision as of 10:03, 27 October 2020
light-on induced system
RelE is from Escherichia coli str. K-12, encoding RelE toxin.
Usage and Biology
In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.
Arabinose induced RelE expression: BBa_K3447104
AHL induced RelE expression: BBa_K3447106
Design
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in GenBank.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1882
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 587
Illegal NgoMIV site found at 659
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 1259
Illegal NgoMIV site found at 1552
Illegal NgoMIV site found at 1646
Illegal AgeI site found at 301
Illegal AgeI site found at 1427 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1316
Illegal BsaI.rc site found at 200