Difference between revisions of "Part:BBa K3447006"
 
Line 4: Line 4:
  
  
−
FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.<br><br>
+
Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
  
−
===Usage and Biology===
 
  
−
We initiated the expression of downstream sfGFP <partinfo>BBa_K2541400</partinfo> and repressor protein CI <partinfo>BBa_C0051</partinfo> by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.<br>
 
−
* Connect sfGFP gene downstream of fixK2 promoter: 链接<br>
 
−
* Connect repressor proteins CI downstream of fixK2 promoter: 链接<br><br>
 
  
 +
===Usage and Biology===
 +
 +
In our project, we use this part together with xynA gene  <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>.
  
 
==<b>Design</b>==
 
==<b>Design</b>==
 
===Design Notes===
 
===Design Notes===
−
We added some synonymous mutations to avoid part rules.
+
There is no need to pay attention.<br>
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
We found this sequence data in the previous iGEM teams (UCAS-China 2018 and Uppsala-Sweden 2011) and in GenBank.<br><br>
+
This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br>
 +
We ordered the part DNA from a synthesis company.<br>
 +
 
  
−
===References===
 
  
 
<!-- -->
 
<!-- -->

Latest revision as of 10:28, 27 October 2020


xynD, which produces xylanase


Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.


Usage and Biology

In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.

Design

Design Notes

There is no need to pay attention.

Source

This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]