Difference between revisions of "Part:BBa K3447005"
 
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T7 promoter is a very specific promoter which is transcribed only by specific T7 RNA polymerase which can activate downstream gene expression after being combined.<br><br>
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Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
  
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===Usage and Biology===
 
  
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In our project, we divided T7 RNA polymerase into two parts, respectively called T7<sub>NT</sub> (链接) and T7<sub>CT</sub> (链接) and attached on the nMag and pMag. Under blue light irradiation, these two parts undergo specific blue light dependent heterodimerization and assemble into complete T7rna polymerase, which acts on PT7 promoter and activates downstream gene expression. And the process is reversible, that is, when blue light is stopped, the dimerized T7RNA polymerase de-dimers and loses its function.<br><br>
 
  
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[[Image: T7 promoter (strong promoter from T7 bacteriophage).jpg|center|frame|100px|<b>Figure 1. </b> Main principles of the molecular mechanism of T7 promoter]]<br><br>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3447005 SequenceAndFeatures</partinfo>
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In our project, we use this part together with xynA gene  <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>.
  
 
==<b>Design</b>==
 
==<b>Design</b>==
 
===Design Notes===
 
===Design Notes===
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We added some synonymous mutations to avoid part rules.
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There is no need to pay attention.<br>
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===Source===
 
===Source===
  
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We found this sequence data in the following reference.<br>
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This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br>
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We ordered the part DNA from a synthesis company.<br>
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*Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, <i>ACS Synth. Biol.</i> 2017, <b>6</b>, 2157-2167.<br><br>
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===References===
 
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1. Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, <i>ACS Synth. Biol.</i> 2017, <b>6</b>, 2157-2167.<br><br>
 
  
  

Latest revision as of 10:30, 27 October 2020

xynD, which produces xylanase


Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.


Usage and Biology

In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.

Design

Design Notes

There is no need to pay attention.

Source

This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.