Difference between revisions of "Part:BBa K3447002:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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There is a repetitive sequence between <i>cvi</i> and <i>cvaC</i> gene, and other than that there is no need to pay attention.
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There is no need to pay attention.
  
 
===Source===
 
===Source===
  
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This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
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We get the genomic sequence by NCBI.<br>
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https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43597&to=43908&strand=true<br>
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−
https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43886&to=44122&strand=true<br>
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−
We ordered the part DNA from a synthesis company.
+
  
 
===References===
 
===References===

Latest revision as of 10:17, 27 October 2020


xynD, which produces xylanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712


Design Notes

There is no need to pay attention.

Source

We get the genomic sequence by NCBI.


References

1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., Journal of Applied Microbiology, 122, 1159-1167.
2. Colicin V can be produced by lactic acid bacteria.(1999), Letters in Applied Microbiology, 29, 37-41.
3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, Plos One, 7(4), e35382.
4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), Biochemistry, 33, 6911-6917.