Difference between revisions of "Part:BBa K3447002"
 
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The <i>cvaC</i> gene produces colicin V, which is also called ColV, is a type of bacteriocin secreted by <i>E. coli</i>. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein, which is synthesized by <i>cvi</i> gene.
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Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
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===Usage and Biology===
 
===Usage and Biology===
  
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the <i>Enterobacteriaceae</i> family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
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In our project, we use this part together with xynA gene <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>.
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The <i>cvaC</i> gene encodes a 103 amino acids' precursor of ColV (preColV), and <i>cvi</i> encodes a protein of 78 amino acids, which can provide immunity against the bactericidal activity of ColV. Therefore, only when the <i>cvaC</i> structural gene and the <i>cvi</i> (237 bp) immune gene exist at the same time, the <i>E. coli</i> itself will not be killed.<br><br>
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We will use cvaC-cvi to express ColV and immunity protein, thereby killing "enemy" bacteria.<br><br>
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==<b>Design</b>==
 
==<b>Design</b>==
 
===Design Notes===
 
===Design Notes===
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There is a repetitive sequence between <i>cvi</i> and <i>cvaC</i> gene, and other than that there is no need to pay attention.<br>
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There is no need to pay attention.<br>
  
 
===Source===
 
===Source===
  
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This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
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This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br>
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*https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43597&to=43908&strand=true<br>
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*https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43886&to=44122&strand=true<br>
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We ordered the part DNA from a synthesis company.<br>
 
We ordered the part DNA from a synthesis company.<br>
  
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===References===
 
===References===
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1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., <i>Journal of Applied Microbiology</i>, <b>122</b>, 1159-1167.<br>
 
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2. Colicin V can be produced by lactic acid bacteria.(1999), <i>Letters in Applied Microbiology</i>, <b>29</b>, 37-41.<br>
 
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3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, <i>Plos One</i>, <b>7(4)</b>, e35382.<br>
 
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4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), <i>Biochemistry</i>, <b>33</b>, 6911-6917.<br>
 

Latest revision as of 10:20, 27 October 2020


xynD, which produces xylanase


Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.


Usage and Biology

In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.

Design

Design Notes

There is no need to pay attention.

Source

This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712



References