Difference between revisions of "Part:BBa K3447000"
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LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. <i>E. coli</i> is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, <i>E. coli</i> is an exception that can sense all kinds of AHL signaling molecules).<br> | LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. <i>E. coli</i> is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, <i>E. coli</i> is an exception that can sense all kinds of AHL signaling molecules).<br> | ||
| − | The constituent produces AHL: | + | The constituent produces AHL: <partinfo>BBa_K3447108</partinfo><br> |
==<b>Design</b>== | ==<b>Design</b>== | ||
Latest revision as of 10:39, 27 October 2020
luxI, an autoinducer synthetase for AHL
LuxI is the synthetase of AHL from Vibrio fischeri.
Usage and Biology
LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. E. coli is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, E. coli is an exception that can sense all kinds of AHL signaling molecules).
The constituent produces AHL: BBa_K3447108
Design
Design Notes
In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.
Source
We get the sequence data on GenBank and we ordered the part DNA from a synthesis company.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]