Difference between revisions of "Part:BBa K3419001"

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===Mechanism===
 
===Mechanism===
 
Upon release into the cell, trichosanthin exhibits RNA N-glycosidase activity and depurinates the 28S rRNA of the 60S eukaryotic ribosomal subunit. This will achieve irreversible inactivation of eukaryotic ribosome activity and inhibit tumor cell function.
 
Upon release into the cell, trichosanthin exhibits RNA N-glycosidase activity and depurinates the 28S rRNA of the 60S eukaryotic ribosomal subunit. This will achieve irreversible inactivation of eukaryotic ribosome activity and inhibit tumor cell function.
[[File:TricosanthinMechanism.png]]
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[[File:TricosanthinMechanism.png|200]]
  
 
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Revision as of 20:17, 17 October 2020


Tricosanthin

Note: All work on this basic part was done virtually through literature research due to COVID-19 restrictions.

Usage and Biology

Trichosanthin is a protein derived from Trichosanthes kirilowii, also known as the Chinese cucumber. It has been used in Eastern traditional medicine as an abortifacient and also functions as an antitumor agent due to its inactivation effect on eukaryotic ribosomes [1]. Trichosanthin is used in our project as an anti-cancer therapeutic protein that selectively kills tumor cells while leaving our engineered prokaryotic E. coli unaffected. We were able to confirm that E. coli would be able to produce this therapeutic based on a literature search (SOURCE #: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1399-3011.1992.tb01558.x). Thus, our bacteria would perform their intended function in our genetic circuit

Mechanism

Upon release into the cell, trichosanthin exhibits RNA N-glycosidase activity and depurinates the 28S rRNA of the 60S eukaryotic ribosomal subunit. This will achieve irreversible inactivation of eukaryotic ribosome activity and inhibit tumor cell function. 200

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 409
    Illegal NgoMIV site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]