Difference between revisions of "Part:BBa K3402061"
 
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===Usage and Biology===
 
===Usage and Biology===
  
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The use of the strongest promoter P<i>tef1</i> to express <i>Cas9</i> protein and sgRNA could increase the gene-editing efficiency. sg<i>PXA1</i> will guide the Cas9 to edit the PXA1 site.
+
The use of the strongest promoter P<i>tef1</i> to express <i>Cas9</i> protein and sgRNA could increase the gene-editing efficiency. sg<i>PXA1</i> will guide the Cas9 to edit the <i>PXA1</i> site.
  
  

Latest revision as of 13:56, 27 October 2020


The Cas9 and sgRNA expression cassette

This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), Tsyn7 (BBa_K3402001) and sgPXA1 (BBa_K3402008).

The Cas9 and sgRNA expression cassette.png

Usage and Biology

The use of the strongest promoter Ptef1 to express Cas9 protein and sgRNA could increase the gene-editing efficiency. sgPXA1 will guide the Cas9 to edit the PXA1 site.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5603
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1730
    Illegal BglII site found at 5294
    Illegal XhoI site found at 1051
    Illegal XhoI site found at 1780
    Illegal XhoI site found at 2143
    Illegal XhoI site found at 5095
    Illegal XhoI site found at 6695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5603
    Illegal NgoMIV site found at 2557
    Illegal NgoMIV site found at 3661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1501
    Illegal BsaI site found at 2218