Difference between revisions of "Part:BBa K3402056"

Revision as of 16:08, 27 October 2020

Single-gene editing cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), sgPXA1(BBa_K3402039), Tsyn7(BBa_K3402001).

Usage and Biology

We achieve the knockout of PXA1 gene and insert the hygromycin resistant gene as a selection marker. Two parts of PXA1 are homologous arms at the ends.
If modified strains can grow on the plate coated with hygromycin, it is successful to knock PXA1 with Cas9 system. By comparison, the editing efficiency of the CRISPR/Cas9 system can be clearly observed.
The single gene-editing efficiency was 100%.

The plates contained hygromycin

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2171
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 04:40, 26 October 2020 (view source) ZZ 0991 (Talk \| contribs) ← Older edit		Revision as of 16:08, 27 October 2020 (view source) ZZ 0991 (Talk \| contribs) Newer edit →
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	The single gene-editing efficiency was 100%.		The single gene-editing efficiency was 100%.

−	[[Image:Single-gene editing cassette.png\|500px\|thumb\|center\|The plates contained hygromycin]]	+	[[Image:Single-gene editing cassette-new.png\|500px\|thumb\|center\|The plates contained hygromycin]]