Difference between revisions of "Part:BBa K3396016"

 
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<partinfo>BBa_K3396016 short</partinfo>
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<partinfo>BBa_K3396008 short</partinfo>
  
 
A dual luciferase reporter to monitor the Fluc tagged protein abundance while rluc can be used to normalize irrelevant factors.
 
A dual luciferase reporter to monitor the Fluc tagged protein abundance while rluc can be used to normalize irrelevant factors.
  
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===Usage and Biology===
 
===Usage and Biology===
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BBa_K3396008 is a Dual Luciferase Reporter system to quantify the abundance of specific target protein. Dual luciferase assay was introduced to normalize the differences caused by irrelevant factors.
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===Special Design===
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Special designs were taken to optimize the applicability and adaptivity of such parts. Specifically, a novel designed substitution system, through which, target proteins could be fused with Fluc fragment using Golden-Gate Assembly, was introduced to dramatically simplify the cloning process.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3396016 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3396008 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3396016 parameters</partinfo>
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<partinfo>BBa_K3396008 parameters</partinfo>
 
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Revision as of 18:30, 26 October 2020


CMV-Replaceable-1 -Fluc-P2A-Rluc

A dual luciferase reporter to monitor the Fluc tagged protein abundance while rluc can be used to normalize irrelevant factors.

Usage and Biology

BBa_K3396008 is a Dual Luciferase Reporter system to quantify the abundance of specific target protein. Dual luciferase assay was introduced to normalize the differences caused by irrelevant factors.

Special Design

Special designs were taken to optimize the applicability and adaptivity of such parts. Specifically, a novel designed substitution system, through which, target proteins could be fused with Fluc fragment using Golden-Gate Assembly, was introduced to dramatically simplify the cloning process.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3482
    Illegal BamHI site found at 4020
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2770
    Illegal NgoMIV site found at 4387
    Illegal NgoMIV site found at 5731
    Illegal NgoMIV site found at 5752
    Illegal AgeI site found at 1615
    Illegal AgeI site found at 5455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721
    Illegal BsaI site found at 2125
    Illegal BsaI site found at 3860
    Illegal BsaI.rc site found at 6618
    Illegal SapI.rc site found at 2473
    Illegal SapI.rc site found at 5637