Difference between revisions of "Part:BBa K3365059"

Revision as of 12:45, 27 October 2020

kanamycin resistant gene with homologous arms

This part is designed to knock out genome rpoZ, which codes for the Omega submit of RNA polymerase. We added 40 base pairs homologous arm at both end of Kanamycin resistant gene in order to replace rpoZ.

Fig:According to the sequencing result, the new sequence, which was rpoZ before, is no longer similar to rpoZ any more, and it is nearly the same sequence as the KanR. This shows that we replace the rpoZ with KanR successfully.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is designed to knock out genome <i>rpoZ</i>, which codes for the Omega submit of RNA polymerase. We added 40 base pairs homologous arm at both end of Kanamycin resistant gene in order to replace <i>rpoZ</i>.
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-===Usage and Biology===
 [[File:Sequencing_result_firstly.png]]
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 <b>Fig:</b>According to the sequencing result, the new sequence, which was <i>rpoZ</i> before, is no longer similar to <i>rpoZ</i> any more, and it is nearly the same sequence as the <i>KanR</i>. This shows that we replace the <i>rpoZ</i> with <i>KanR</i> successfully.
+<!--
+===Usage and Biology===
 <!-- -->