Difference between revisions of "Part:BBa K3365054"
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<partinfo>BBa_K3365054 short</partinfo> | <partinfo>BBa_K3365054 short</partinfo> | ||
| − | This part is used as a reporter for detecting whether dCas9-ωis off-target. We | + | This part is used as a reporter for detecting whether dCas9-ωis off-target. We inserted this part downstream of transcription activating unit of each lure sequence. If dCas9-ω is binding to the lure sequence, the expression of this part will be activated and the cell then emit green fluorescence, allowing us to know that dCas9-ωis off-target. As we plan to put the potential off-target site one by one, we added a terminator to eGFP so that once binding, the dCas9-ωcan only activating the closest reporter’s expression downstream. |
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Latest revision as of 12:57, 27 October 2020
enhanced GFP with double terminator
This part is used as a reporter for detecting whether dCas9-ωis off-target. We inserted this part downstream of transcription activating unit of each lure sequence. If dCas9-ω is binding to the lure sequence, the expression of this part will be activated and the cell then emit green fluorescence, allowing us to know that dCas9-ωis off-target. As we plan to put the potential off-target site one by one, we added a terminator to eGFP so that once binding, the dCas9-ωcan only activating the closest reporter’s expression downstream.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
Illegal XhoI site found at 730 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]