Difference between revisions of "Part:BBa K3365053:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
  
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Be careful especially when designing primers. Due to complex secondary structure of double terminator, we keep a small insignificant sequence downstream of double terminator as linker for convenient when amplifying this part by PCR.
+
Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR.
  
 
===Source===
 
===Source===
  
−
We amplify this part on plasmid pE1a-RFP kindly offered by team Tongji_China.
+
We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China.
  
 
===References===
 
===References===

Latest revision as of 12:56, 27 October 2020


RFP with double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 700
    Illegal XhoI site found at 691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR.

Source

We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China.

References