Difference between revisions of "Part:BBa K3282002"
 
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This part contains coding sequence of ''Cupriavidus metallidurans'' CH34  lead binding protein (''pbrD'', Gene ID: 3170401, Uniprot: Q58AJ8) codon-optimized for Escherichia coli Nissle 1917. Pb(II) resistance was early recognized to be located on pMOL30, one of the two plasmids found in ''Cupriavidus metallidurans'' CH34 [1]. The ''pbrD'' gene encodes a Pb(II)-binding protein which is essential for functional lead sequestration. When Pb(II) enters the cytoplasm, it can be bound by the ''pbrD'' protein, which may function as a chaperone for Pb(II) and cells lacking ''pbrD'' show a decreased accumulation of Pb(II) and this accumulation may protect against free exported Pb(II) [3].
 
This part contains coding sequence of ''Cupriavidus metallidurans'' CH34  lead binding protein (''pbrD'', Gene ID: 3170401, Uniprot: Q58AJ8) codon-optimized for Escherichia coli Nissle 1917. Pb(II) resistance was early recognized to be located on pMOL30, one of the two plasmids found in ''Cupriavidus metallidurans'' CH34 [1]. The ''pbrD'' gene encodes a Pb(II)-binding protein which is essential for functional lead sequestration. When Pb(II) enters the cytoplasm, it can be bound by the ''pbrD'' protein, which may function as a chaperone for Pb(II) and cells lacking ''pbrD'' show a decreased accumulation of Pb(II) and this accumulation may protect against free exported Pb(II) [3].
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The part was evaluated in the composite parts  [https://parts.igem.org/Part:BBa_K3282005 (Part:BBa_K3282005)]and [https://parts.igem.org/Part:BBa_K3282007 (Part:BBa_K3282007)] expressed in pUC19 backbone (part---). The composites have the same RBS [https://parts.igem.org/Part:BBa_J61109 (Part:BBa_J61109)] and terminators [https://parts.igem.org/Part:BBa_B0012 (Part:BBa_B0012)] with two different promoters T7 [https://parts.igem.org/Part:BBa_I719005 (Part:BBa_I719005)] and Tac [https://parts.igem.org/Part:BBa_K3282006 (Part:BBa_K3282006)]. The four composite parts were tested to determine their Lead accumulation capability.
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The part was evaluated in the composite parts  [https://parts.igem.org/Part:BBa_K3282005 (Part:BBa_K3282005)]and [https://parts.igem.org/Part:BBa_K3282007 (Part:BBa_K3282007)] expressed in pUC19 backbone. The composites have the same RBS [https://parts.igem.org/Part:BBa_J61109 (Part:BBa_J61109)] and terminators [https://parts.igem.org/Part:BBa_B0012 (Part:BBa_B0012)] with two different promoters T7 [https://parts.igem.org/Part:BBa_I719005 (Part:BBa_I719005)] and Tac [https://parts.igem.org/Part:BBa_K3282006 (Part:BBa_K3282006)]. The four composite parts were tested to determine their Lead accumulation capability.
  
  

Latest revision as of 16:40, 17 October 2019


C. metallidurans lead binding protein - pbrD

This part contains coding sequence of Cupriavidus metallidurans CH34 lead binding protein (pbrD, Gene ID: 3170401, Uniprot: Q58AJ8) codon-optimized for Escherichia coli Nissle 1917. Pb(II) resistance was early recognized to be located on pMOL30, one of the two plasmids found in Cupriavidus metallidurans CH34 [1]. The pbrD gene encodes a Pb(II)-binding protein which is essential for functional lead sequestration. When Pb(II) enters the cytoplasm, it can be bound by the pbrD protein, which may function as a chaperone for Pb(II) and cells lacking pbrD show a decreased accumulation of Pb(II) and this accumulation may protect against free exported Pb(II) [3]. The part was evaluated in the composite parts (Part:BBa_K3282005)and (Part:BBa_K3282007) expressed in pUC19 backbone. The composites have the same RBS (Part:BBa_J61109) and terminators (Part:BBa_B0012) with two different promoters T7 (Part:BBa_I719005) and Tac (Part:BBa_K3282006). The four composite parts were tested to determine their Lead accumulation capability.


1. Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., & Lelie, D. V. D. (2008). Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek, 96(2), 171–182. doi: 10.1007/s10482-008-9289-0.

2.Hobman, J. L., Julian, D. J., & Brown, N. L. (2012). Cysteine coordination of Pb(II) is involved in the PbrR-dependent activation of the lead-resistance promoter, PpbrA, from Cupriavidus metallidurans CH34. BMC Microbiology, 12(1), 109. doi: 10.1186/1471-2180-12-109


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]