Difference between revisions of "Part:BBa K3257047"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3257047 short</partinfo>
 
<partinfo>BBa_K3257047 short</partinfo>
  
 
This part is a truncated mCherry and it only contains amino acid residues from the1<sup>st</sup> to the 158<sup>th</sup> of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.
 
This part is a truncated mCherry and it only contains amino acid residues from the1<sup>st</sup> to the 158<sup>th</sup> of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.
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[[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X  represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside ''E.coli'' cells.]]
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257047 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257047 SequenceAndFeatures</partinfo>
  

Revision as of 21:23, 21 October 2019

Truncated mCherry

This part is a truncated mCherry and it only contains amino acid residues from the1st to the 158th of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.

Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside E.coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]