Difference between revisions of "Part:BBa K3239011:Experience"
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===Applications of BBa_K3239011=== | ===Applications of BBa_K3239011=== | ||
| − | This construct is designed to appropriately up-regulate the AOX1 promoter ( | + | This construct is designed to appropriately up-regulate the ''AOX1'' promoter (''P<sub>AOX1'') activity in ''P. pastoris GS115'' at a high yet tolerable level. Both sub-parts are to be integrated into the ''P. pastoris GS115 strain'' separately. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 07:16, 18 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3239011
This construct is designed to appropriately up-regulate the AOX1 promoter (PAOX1) activity in P. pastoris GS115 at a high yet tolerable level. Both sub-parts are to be integrated into the P. pastoris GS115 strain separately.
User Reviews
We labeled our strains containing this construct pGMP1-pAPM1-PAOX1-GFP. We just acquired these strains and need to conduct more experiments to draw more conclusive results. Our modeling, however, has demonstrated that this construct should very strongly upregulate PAOX1 activity.
For detailed modeling and design, please check our wiki:
https://2019.igem.org/Team:ShanghaiFLS_China/Model
https://2019.igem.org/Team:ShanghaiFLS_China/Design
UNIQ2626c46cb4c3ec82-partinfo-00000000-QINU
UNIQ2626c46cb4c3ec82-partinfo-00000001-QINU