Difference between revisions of "Part:BBa K3239011:Experience"

Revision as of 07:15, 18 October 2019

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3239011

This construct is designed to appropriately up-regulate the AOX1 promoter (pAOX1) activity in P. pastoris GS115 at a high yet tolerable. Both sub-parts are to be homologously reintegrated into the P. pastoris GS115 strain.

User Reviews

We labeled our strains containing this construct pGMP1-pAPM1-P_AOX1-GFP. We just acquired these strains and need to conduct more experiments to draw more conclusive results. Our modeling, however, has demonstrated that this construct should very strongly upregulate P_AOX1 activity.

For detailed modeling and design, please check our wiki:

https://2019.igem.org/Team:ShanghaiFLS_China/Model

https://2019.igem.org/Team:ShanghaiFLS_China/Design

UNIQ2be6495d7563fc6d-partinfo-00000000-QINU UNIQ2be6495d7563fc6d-partinfo-00000001-QINU

@@ Line 8: / Line 8: @@
 ===User Reviews===
-We labeled our strains containing this construct pGMP1-pAPM1-pAOX1-GFP. We just acquired these strains and are planning to conduct relevant experiments. Our modeling, however, has demonstrated that this construct should very strongly upregulate pAOX1 activity.
+We labeled our strains containing this construct ''pGMP1''-''pAPM1''-''P<sub>AOX1''-GFP. We just acquired these strains and need to conduct more experiments to draw more conclusive results. Our modeling, however, has demonstrated that this construct should very strongly upregulate ''P<sub>AOX1'' activity.
+For detailed modeling and design, please check our wiki:
+https://2019.igem.org/Team:ShanghaiFLS_China/Model
+https://2019.igem.org/Team:ShanghaiFLS_China/Design
 <!-- DON'T DELETE --><partinfo>BBa_K3239011 StartReviews</partinfo>