Difference between revisions of "Part:BBa K3239011"

Revision as of 07:03, 18 October 2019

pMIT1-PRM1-pPRM1-MIT1

A combination of the pMIT1-PRM1 and the pPRM1-MIT1 constructs.

Usage and Biology

This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the proposed complex regulation of P_AOX1.

Figure 1. Illustration of the pGMP-pAPM1-P_AOX1-GFP construct (for in trans regulations among homogeneous transcription factors and the homogeneous AOX1, see the P_AOX1-GFP construct in part BBa_K3239007

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556
Illegal PstI site found at 6970
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2034
Illegal NheI site found at 3815
Illegal SpeI site found at 793
Illegal PstI site found at 2556
Illegal PstI site found at 6970
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2034
Illegal BglII site found at 1387
Illegal BglII site found at 4268
Illegal BglII site found at 7428
Illegal BamHI site found at 2917
Illegal BamHI site found at 3836
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556
Illegal PstI site found at 6970
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556
Illegal PstI site found at 6970
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
 This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the proposed complex regulation of ''P<sub>AOX1''.
-[[File:pGMP1-pAPM1-pAOX1-GFP.png|400px|thumb|Figure 1. Illustration of the ''pGMP''-''pAPM1''-''P<sub>AOX1''-GFP construct (for in trans regulations among homogeneous transcription factors and the homogeneous ''AOX1'', see the ''P<sub>AOX1''-GFP construct in part BBa_K3239007]]
+[[File:pGMP1-pAPM1-pAOX1-GFP.png|400px|thumb|Figure 1. Illustration of the ''pGMP''-''pAPM1''-''P<sub>AOX1''-GFP construct (for ''in trans'' regulations among homogeneous transcription factors and the homogeneous ''AOX1'', see the ''P<sub>AOX1''-GFP construct in part BBa_K3239007]]