Difference between revisions of "Part:BBa K323153"

Revision as of 16:20, 27 October 2010

Scrambled DNA program

This part contains a DNA program - a specifically designed nucleotide sequence containing 6 zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger domains) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Zif268 - PBSII - Jazz - Blues - HivC - Gli1.

We have bound five chimeric enzymes, encoded by Part:BBa_K323135 and Part:BBa_K323132 to the program to show that a correct arrangement of enzymes on the DNA program is important for the progress of the reaction.

[[Image:Slovioscr.png|center|thumb|500px| Figure: Chimeric enzymes for synthesis violacein bound on the scrambled DNA progam.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K323153 short</partinfo>
-Scrambled DNA program, used as a control of the importance of seguence of binding sites.
+This part contains a DNA program - a specifically designed nucleotide sequence containing 6 zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger domains) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Zif268 - PBSII - Jazz - Blues - HivC - Gli1.
-repetitions, also contains junk DNA.
+We have bound five chimeric enzymes, encoded by Part:BBa_K323135 and Part:BBa_K323132 to the program to show that a correct arrangement of enzymes on the DNA program is important for the progress of the reaction.
+[[Image:Slovioscr.png|center|thumb|500px| Figure: Chimeric enzymes for synthesis violacein bound on the scrambled DNA progam.
 <!-- Add more about the biology of this part here