Difference between revisions of "Part:BBa K3226019:Design"

(→‎Design Notes)
(→‎References)
 
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===References===
 
===References===
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Dea Slade1,and Miroslav Radman,Oxidative Stress Resistance in Deinococcus radiodurans, Microbiol Mol Biol Rev. 2011 Mar; 75(1): 133–191.

Latest revision as of 15:23, 17 October 2019


RecA+his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence.

Source

Deinococcus Radioduranse

References

Dea Slade1,and Miroslav Radman,Oxidative Stress Resistance in Deinococcus radiodurans, Microbiol Mol Biol Rev. 2011 Mar; 75(1): 133–191.