Difference between revisions of "Part:BBa K321004"
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In wild-type NK cells, Killer cell immunoglobulin-like receptors (KIRs) with  long cytoplasmic domains inhibit cell mediated cytotoxicity upon ligand binding. They possess a immune tyrosine-based inhibitory motif (ITIM) that when phosphorylated recruits phosphotases like SHP-1 that decrease the activation molecules involved in cell signaling.  
 
In wild-type NK cells, Killer cell immunoglobulin-like receptors (KIRs) with  long cytoplasmic domains inhibit cell mediated cytotoxicity upon ligand binding. They possess a immune tyrosine-based inhibitory motif (ITIM) that when phosphorylated recruits phosphotases like SHP-1 that decrease the activation molecules involved in cell signaling.  
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===Function===
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This part was shown to inhibit the activation of killer cells. We use this part in our ANDN Gate to increase the precision of killing toward cancer cells. The image below shows the inhibitory function of this part. For more information please click <html><a href="http://2010.igem.org/Team:UCSF/Project/Precision#andn">Here</a></html>
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<img src="https://static.igem.org/mediawiki/2010/8/80/UCSF_ANDNgate_results.png"/>
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</html>
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Revision as of 03:06, 28 October 2010

intracellular chain of KIR3DL1

Encodes the intracellular signaling chain of the KIR3DL1 immune receptor. Inhibits immune cell activation via the ITIM (immunoreceptor tyrosine-based inhibition motif) motif.


Usage and Biology

In wild-type NK cells, Killer cell immunoglobulin-like receptors (KIRs) with long cytoplasmic domains inhibit cell mediated cytotoxicity upon ligand binding. They possess a immune tyrosine-based inhibitory motif (ITIM) that when phosphorylated recruits phosphotases like SHP-1 that decrease the activation molecules involved in cell signaling.


Function

This part was shown to inhibit the activation of killer cells. We use this part in our ANDN Gate to increase the precision of killing toward cancer cells. The image below shows the inhibitory function of this part. For more information please click Here




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]