Difference between revisions of "Part:BBa K3182009"

Revision as of 13:19, 19 October 2019

Parseq-α

Parseq alpha is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria was combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD⁶⁰⁰ was measured. The results can be seen in Figure 1.. Parseq alpha showed an inhibiting effect on the growth of E.coli. A concentration of 30 ng/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.

Figure 1 Shows the antimicrobial activity of Parseq Alpha on A) E.Coli. and B) B.subtilis. The samples were cultured in 200 µL, in a 96 well plate and incubated at 37°C. Shaking was conducted for 1 minute every 15 minutes and the OD⁶⁰⁰ was measured every hour for 16 hours. The negative control represents the bacterial growth with no antimicrobial peptides present. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3182009 short</partinfo>
-Parseq alpha is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea do to there hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptide in different concentration. they were then incubated at 37°C with shaking every 15 minutes. the plate was incubated for 16 hours and OD<sup>600</sup> was measured. The results from this can be seen in <b>Figure 1.</b>. Parseq alpha showed an inhibiting effect on the growth of E.coli. 30 ng/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.
+Parseq alpha is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria was combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD<sup>600</sup> was measured. The results can be seen in <b>Figure 1.</b>. Parseq alpha showed an inhibiting effect on the growth of E.coli. A concentration of 30 ng/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.
 [[File:T--Linkoping_Sweden--AlphaEcoli.png|650px|thumb|center|<b>Figure 1</b> Shows the antimicrobial activity of Parseq Alpha
-  on A) <i>E.Coli.</i> and B) <i>B.subtilis</i>. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C. shaking was conducted for 1 minute every 15 minutes and the OD<sup>600</sup> were messured every hour for 16 hours. the negative control represents the bacterial growth with no antimicrobial peptides precent. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad ]]
+  on A) <i>E.Coli.</i> and B) <i>B.subtilis</i>. The samples were cultured in 200 µL, in a 96 well plate and incubated at 37°C. Shaking was conducted for 1 minute every 15 minutes and the OD<sup>600</sup> was measured every hour for 16 hours. The negative control represents the bacterial growth with no antimicrobial peptides present. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad ]]