Difference between revisions of "Part:BBa K3142012"
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| − | + | Low glucose environments cause the cAMP-CRP complex to bind target DNA and regulate hundreds of gene targets. [1] There are 3 classes of CRP promoters, categorized by the number and placement of CRP binding sites within each. Class I promoters have one CRP binding site at various distances upstream of the -35 box which interact with the α-C Terminal Domain (α-CTD) of RNA Polymerase (RNAP) . [16]Class II promoters have CRP encompassing the -35 box, and interact with α-CTD and α-NTD of RNAP for promoter recruiting .[ 17] This site partially occludes the σ70 subunit of RNAP, and is likely why multiple interactions with cAMP-CRP are made. Class III promoters contain a mix of multiple Class I and II CRP sites. [16] | |
| + | Three versions of each promoter were generated: (1) “full length” promoters (crp) starting ~ 500 bp upstream of the open reading frame and ending at the transcription start site (2) minimal “truncated” promoters (T-crp) containing ~ 50 bp upstream of the transcription start site with care taken to include the full CRP binding site, and (3) enhanced truncated promoters (T-αcrp) which include a modified enhancer element from the CC(-41.5)α(-63) synthetic elements upstream of the T-crp promoters. 20 | ||
| − | what it | + | '''what it is'''：Glucose starved promoter |
| − | how to use it in their projects | + | '''what it does'''：Response to glucose starvation |
| + | |||
| + | '''how to use it in their projects'''：Initiating the autolysis gene expressing lactic acid bacteria in response to glucose starvation | ||
Revision as of 01:23, 21 October 2019
Glucose starvation promoter（PT-αcrp）
Low glucose environments cause the cAMP-CRP complex to bind target DNA and regulate hundreds of gene targets. [1] There are 3 classes of CRP promoters, categorized by the number and placement of CRP binding sites within each. Class I promoters have one CRP binding site at various distances upstream of the -35 box which interact with the α-C Terminal Domain (α-CTD) of RNA Polymerase (RNAP) . [16]Class II promoters have CRP encompassing the -35 box, and interact with α-CTD and α-NTD of RNAP for promoter recruiting .[ 17] This site partially occludes the σ70 subunit of RNAP, and is likely why multiple interactions with cAMP-CRP are made. Class III promoters contain a mix of multiple Class I and II CRP sites. [16] Three versions of each promoter were generated: (1) “full length” promoters (crp) starting ~ 500 bp upstream of the open reading frame and ending at the transcription start site (2) minimal “truncated” promoters (T-crp) containing ~ 50 bp upstream of the transcription start site with care taken to include the full CRP binding site, and (3) enhanced truncated promoters (T-αcrp) which include a modified enhancer element from the CC(-41.5)α(-63) synthetic elements upstream of the T-crp promoters. 20
what it is：Glucose starved promoter
what it does：Response to glucose starvation
how to use it in their projects：Initiating the autolysis gene expressing lactic acid bacteria in response to glucose starvation
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 26
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 26
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 26
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
SZPT-China
To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.
