Difference between revisions of "Part:BBa K3128021:Design"

 
 
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Homodimerization is only possible if two leucine zipper proteins meet.
 
Homodimerization is only possible if two leucine zipper proteins meet.
  
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+
In iGEM Grenoble 2019 project, we used leucine zipper to get closer two OMPx proteins. Indeed, we wanted to verify if when the two proteins are closed together, we can have a signal. For that, we created a fusion protein with OMPx and leucine zipper as you can see on BBa_K3128026 and BBa_K3128027.
  
 
===Source===
 
===Source===

Latest revision as of 13:14, 13 October 2019


Leucine Zipper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 41


Design Notes

Homodimerization is only possible if two leucine zipper proteins meet.

In iGEM Grenoble 2019 project, we used leucine zipper to get closer two OMPx proteins. Indeed, we wanted to verify if when the two proteins are closed together, we can have a signal. For that, we created a fusion protein with OMPx and leucine zipper as you can see on BBa_K3128026 and BBa_K3128027.

Source

This gene was synthesized by iDT because it was non available on iGEM plates.

References