Difference between revisions of "Part:BBa K3110003:Design"
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===Design Notes=== | ===Design Notes=== | ||
The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD. | The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 10:47, 21 October 2019
lldP + lldR + lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 275
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3597
Illegal AgeI site found at 411
Illegal AgeI site found at 874
Illegal AgeI site found at 1312
Illegal AgeI site found at 1471
Illegal AgeI site found at 1483
Illegal AgeI site found at 2229 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3034
Design Notes
The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.
Source
lldD was synthesized by Twist Biosciences and lldP + lldR was obtained from the genomic DNA of Ecoli Dh5α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).