Difference between revisions of "Part:BBa K3105677:Design"

 
 
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===Design Notes===
 
===Design Notes===
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AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the expression system. α-factor secretion signal was used due to  
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AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the target protein. <br>
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&#945;-factor secretion signal was used to enable secretion of target enzyme for easy purification from the supernatant.
  
  

Latest revision as of 23:07, 19 October 2019


Expression construct HRP-2A-eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2086
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2167
    Illegal AgeI site found at 3176
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the target protein.
α-factor secretion signal was used to enable secretion of target enzyme for easy purification from the supernatant.


Source

See basic parts

References