Difference between revisions of "Part:BBa K3071012"

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<center><u>Figure 3  rt-qPCR data Azathioprine treatment on the eforRED mRNA expression level </u></center>
 
<center><u>Figure 3  rt-qPCR data Azathioprine treatment on the eforRED mRNA expression level </u></center>
 
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Azathioprine is an indirect supressor to cyclic-di-GMP, treatment of azathioprine to bacteria culture can reduce the cyclic-di-GMP level and lead to activation of the pspF-Clp and by-pass the RpfC/RpfG two-component system.The result from rt-qPCR data shows that the above sysntehtic biological system is functional with significant up-regulation of reporter mRNA.
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Azathioprine is an indirect supressor to cyclic-di-GMP, treatment of azathioprine to bacteria culture can reduce the cyclic-di-GMP level and lead to activation of the pspF-Clp and by-pass the RpfC/RpfG two-component system.The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA.
 
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<center>[[file:T--Hong_Kong-CUHK--DSF_assay.png]]</center>
 
<center>[[file:T--Hong_Kong-CUHK--DSF_assay.png]]</center>
 
<center><u>Figure 4  rt-qPCR data DSF treatment on the eforRED mRNA expression level</u></center>
 
<center><u>Figure 4  rt-qPCR data DSF treatment on the eforRED mRNA expression level</u></center>
 
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The result from rt-qPCR data shows that the above sysntehtic biological system is functional with significant up-regulation of reporter mRNA upon DSF activation.  
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The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA upon DSF activation.  
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 16:42, 21 October 2019


Clp-binding site II from gumB operon (CBS II)

Description

This is the regulatory element from the gum operon of Xanthomonas campestris pv. camperstris. The sequence is centered in the nt -93.5 of gumB upstream sequence.

Biology

T--Hong Kong-CUHK--CBSI and II sequence.png
Figure 1 Basic properties of gumB UAS


Clp binding sites (CBSs) have typically been identified by pattern searching of the Xanthomonas campestris pv. camperstris genome using the consensus CRP binding sequence.Clp upregulates the gum operon by binding to two non-consensus sites. CBS I has a high GC content in the central region (6bp) that may be important for binding, and binding may be enhanced if the GC-rich central region is palindromic.

Usage

T--Hong Kong-CUHK--whole design.png
Figure 2 illustration of our synthetic biological system upon DSF activation


This regulatory element is used to construct our synthetic reporter construct (BBa_K3071024). It is the DNA sequence that could interact with pspF-Clp (BBa_K3071009) and lead to the activation of synthetic promoter (BBa_K3071014).

Characterization

T--Hong Kong-CUHK--Azathioprine treated rt-qPCR data.png
Figure 3 rt-qPCR data Azathioprine treatment on the eforRED mRNA expression level


Azathioprine is an indirect supressor to cyclic-di-GMP, treatment of azathioprine to bacteria culture can reduce the cyclic-di-GMP level and lead to activation of the pspF-Clp and by-pass the RpfC/RpfG two-component system.The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA.

T--Hong Kong-CUHK--DSF assay.png
Figure 4 rt-qPCR data DSF treatment on the eforRED mRNA expression level


The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA upon DSF activation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]