Difference between revisions of "Part:BBa K3056001:Design"

 
(→‎References)
 
Line 18: Line 18:
  
 
===References===
 
===References===
 +
1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019)

Latest revision as of 20:12, 11 October 2019


Anti-THC antibody fragment (ScFv), bound to EGFP at the C-terminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1380
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


Design Notes

EGFP was chosen over other fluorescent protein, as it is brighter than most.


Source

The antibody fragment was obtained from "The Production of Recombinant single chain Antibody Fragments for the Detection of Illicit Drug Residues", written by Dr. Brennan.

References

1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019)