Difference between revisions of "Part:BBa K3016210:Experience"
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===Applications of BBa_K3016210=== | ===Applications of BBa_K3016210=== | ||
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| + | ==Use by Aalto-Helsinki 2019== | ||
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| + | Aalto-Helsinki attempted to characterize this part in <i>Vibrio natriegens</i> by cloning and expressing it. We were unsuccessful as we found the CDS always mutated during the cloning phase. We used a vector with a low copynumber p15A ori and a tight pRhaB promoter, with which we had no problems when working with other genes (YGFP and TfoX). This implies that the gene is extremely toxic to <i>Vibrio natriegens</i> cells. | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 19:04, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3016210
Use by Aalto-Helsinki 2019
Aalto-Helsinki attempted to characterize this part in Vibrio natriegens by cloning and expressing it. We were unsuccessful as we found the CDS always mutated during the cloning phase. We used a vector with a low copynumber p15A ori and a tight pRhaB promoter, with which we had no problems when working with other genes (YGFP and TfoX). This implies that the gene is extremely toxic to Vibrio natriegens cells.
User Reviews
UNIQ5a6b734909b83071-partinfo-00000000-QINU UNIQ5a6b734909b83071-partinfo-00000001-QINU