Difference between revisions of "Part:BBa K3016100"

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Aalto-Helsinki 2019 used this part by combining it with YGFP ([https://parts.igem.org/Part:BBa_K3016600 BBa_K3016600]), a slow-bleaching GFP variant, creating a composite TorA-YGFP part ([https://parts.igem.org/Part:BBa_K3016200 BBa_K3016200]) to test protein translocation into <i>Vibrio natriegens'</i> and <i>Escherichia coli's</i> periplasm.  
 
Aalto-Helsinki 2019 used this part by combining it with YGFP ([https://parts.igem.org/Part:BBa_K3016600 BBa_K3016600]), a slow-bleaching GFP variant, creating a composite TorA-YGFP part ([https://parts.igem.org/Part:BBa_K3016200 BBa_K3016200]) to test protein translocation into <i>Vibrio natriegens'</i> and <i>Escherichia coli's</i> periplasm.  
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Revision as of 20:46, 20 October 2019


Vibrio natriegens' TorA signal peptide


This part contains Vibrio natriegens' TorA Tat signal peptide. It is a twin-arginine (RR) motif containing signal peptide for periplasmic transport of proteins via the twin-arginine translocation (Tat) pathway. Derived from Vibrio natriegens’ torA gene.


Biology

The twin-arginine translocation (Tat) pathway is capable of translocating fully folded proteins up to 150 kDa. It also contains a quality control feature of rejecting misfolded proteins. In some cases, disulfide bridge formation is not required for successful translocation. (Alanen et al., 2015)

Translocation using the tat-pathway requires the protein to contain a N-terminal signal peptide with a twin-arginine (RR) motif. The signal peptide is cleaved during the translocation process. A pair of V. natriegens’ native twin-arginine signal peptides identified by Aalto-Helsinki can be found here (TorAand Aminotransferase)

Use

This part can be N-terminally fused with your protein of choice to facilitate its periplasmic transport via the twin-arginine translocation (Tat) pathway.

Aalto-Helsinki 2019 used this part by combining it with YGFP (BBa_K3016600), a slow-bleaching GFP variant, creating a composite TorA-YGFP part (BBa_K3016200) to test protein translocation into Vibrio natriegens' and Escherichia coli's periplasm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]